Many companies are increasing their interest in bioethanol due to the need for renewable liquid fuels most specifically in the transport industry. Due to its richness in crude protein, vitamins, fat, minerals, and fibers, it is presently used as a feed in aquaculture, livestock, and poultry. In present years, these grains have been used as feedstock in the production of value-added products through microbial fermentation. Many studies tell about the production of organic acids, biohydrogen, methane, and hydrolytic enzymes using DDGS. While it also contains remarkable amounts of macronutrients. A pre-treatment of this product is required for the release of the fermentable sugars. The pre-treatment techniques like physical, chemical, and biological origin are either used individually or combined to obtain maximum results for different applications.